First report of beet curly top Iran virus infecting cantaloupe in Iran

Document Type : Short scientific report

Authors

Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman 7616914111, Kerman/IRAN.

Abstract

The family Geminiviridae comprises a large group of plant-infecting viruses with twined particles and one or two molecules of circular single-stranded DNA genome (Navas-Castillo & Fiallo-Olivé 2021). During a survey for identification of geminivirus-infecting crops in 2017, diseased cantaloupe plants (Cucumis melo L. subsp. melo) (n=10) showing cup-shaped and crinkling leaves symptoms (Fig. 1) were sampled from Sabzevar farms in the west of Khorasan Razavi Province, Iran. Total DNA were extracted from diseased samples using a CTAB method followed by enrichment of circular DNA molecules of the samples using rolling circle amplification (RCA) method and phi29 DNA polymerase enzyme (TempliPhi, GE Healthcare, USA) as previously described by Shepherd et al. (2008). Subsequently, restriction pattern of the samples was analyzed using different enzymes. Due to identical restriction pattern of the resulted digested RCA product, the sample Ta-109 was chosen and HindIII restricted RCA product (~2.9 kb in size) was cloned into the HindIII digested pGreen0029 plasmid followed by Sanger sequencing of a part (976 bp) of the recombinant plasmid. The resulted sequence of the isolate Ta-109 was used to search the database using basic local alignment search tool (BLASTn) (Altschul et al., 1990). Results indicated that beet curly top Iran virus (Becurtovirus betae, family Geminiviridae) is associated with the cantaloupe sample.

Keywords

Journal of Advances in Plant Protection
Volume 2, Issue 1
June 2025
Pages 79-80

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